By R.L.M Pierik
In Vitro tradition of upper Plants offers an up to date and broad- ranging account of the ideas and functions, and has basically been written in line with useful difficulties. distinctive consciousness has been paid to the academic elements.
usual methodological facets are given within the first half: laboratory set-up, composition and education of media, sterilization of media and plant fabric, isolation and (sub)culture, mechanization, the effect of plant and environmental components on progress and improvement, the move from test-tube to soil, aids to check. The query of why in vitro tradition is practised is roofed within the moment half: embryo tradition, germination of orchid seeds, mericloning of orchids, creation of disease-free vegetation, vegetative propagation, somaclonal version, test-tube fertilization, haploids, genetic manipulation, different functions in phytopathology and plant breeding, secondary metabolites.
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Extra info for In Vitro Culture of Higher Plants
1974 Regeneration of haploid Petunia hybrida plants from protoplasts (Binding). 1974 Fusion of haploid protoplasts found possible which gave rise to hybrids (Melchers and Labib). 1974 Biotransformation in plant tissue cultures (Reinhard). ). 1975 Positive selection of maize callus cultures resistant to Helminthosporium maydis (Gengenbach en Green). 24 1976 Shoot initiation from cryo-preserved shoot apIces of carnation (Seibert). ). ). ). ). ). ). 1981 Introduction of the term somaclonal variation (Larkin and Scowcroft).
3. Flasks containing 500-5,000 ml nutrient media: 35 min. at 121°C. 4. Empty test tubes, flasks and filter paper: 30 min. at 130°C. For safety, when autoclaving bottles, they should not be too tightly packed and their tops should be loose. ) require 2-3 h dry sterilization at 160°C. As has already become clear, nutrient media and' empty objects', such as glass, paper, etc. should be sterilized separately. The same holds true for large and small flasks. It must be realized that the heat penetration is very important in an autoclave; and large volumes must in principle be sterilized for longer periods, as the heat will take longer to penetrate than with smaller volumes.
Takes place on a sterilized glass plate or between/on sterile filter paper, it is still necessary to carry out these tasks in a laminar air-flow cabinet (also called an inoculation cabinet). g. in the case of large pratical classes where the use of laminar air-flow cabinets would be too expensive) at least lO % infection must be expected. Research laboratories and commercial tissue culture laboratories always use laminar airflow cabinets to limit the possibilities of infection. A laminar air-flow cabinet (Fig.
In Vitro Culture of Higher Plants by R.L.M Pierik