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Papers dried in an oven tend not to stain well with protein dyes. As haemoglobin is a coloured solution the electrophoretogram can be read visually but staining is recommended as it is possible to miss very small quantities of abnormal haemoglobin which may be present. The protein stains described on p. 10 are suitable. Alternatively, photography, as described on p. 155 is also useful. For details of staining techniques for haemoglobins see p. 51. (Fig.

Clin. , 1957, 16, 75. 15. Harris, H . , P e n r o s e , L. , and T h o m a s , D . H . H . Ann. Hum. , L o n d o n . 1959, 2 3 , 442. PAPER ELECTROPHORESIS AT LOW VOLTAGE 35 16. B l a s s , J . , a n d R e b e y r o t t e , P . Bull. Soc. Chim. , P a r i s , 1953, 3 5 , 953. 17. B r o n k , J . , a n d Fisher, R. B . Biochem. J . , 1956, 6 4 , 106. 18. S m i t h , J . D . in The Nucleic Acids, Vol. 1. p . 267, A c a d e m i c Press, N e w York, 1955. 19. D a v i d s o n , J . , a n d Smellie, R .

Current. 0-3 mA/cm. width of the paper. Voltage. Approximately 180 V. Time of Run. 16 to 18 hours. Drying and Staining. The same as for the horizontal method, except that drjdng may take up to 6 hours. Figs. 6 show photo­ graphs of stained and unstained electrophoretograms obtained by the vertical method. s F I G . 5. P h o t o g r a p h s of u n s t a i n e d paper electrophoretograms s h o w i n g N o r m a l Haemoglobin a n d a n AC a n d A S trait (Vertical method). 46 ELECTROPHORETIC TECHNIQUES F I G .

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Accelerate Your Keyboard Playing by Dave Ddhlp 00320460 Limina

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